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KMID : 1147820150040010044
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Journal of Naturopathy
2015 Volume.4 No. 1 p.44 ~ p.52
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Cloning and Genetic Analysis of Invasion Genes invH, invF and hilA of Salmonella enteritidis and S. typhimurium
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Hong Ji-Ae
Kim Jin-Woo
Na Hun-Taek
Kim Soo-Young
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Abstract
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In this study, a gene sequence was determined after cloning genes invH, invF and hilA from Salmonella enterica subsp. enterica, serovar typhimurium and enteritidis. Genes were identified using electrophoresis after being PCR conducted. The result of comparison of determined gene sequence with Salmonella typhimurium LT2 and S. enterica U84273.1 (S. enterica subsp. enterica) showed highly correlated characteristics, i.e,. 99% for invH gene, and 100% for genes invF and hilA, respectively. Finally, in this study was formed a library of invasion, which is known as a toxicity factor of Salmonella, and determined the candidate genes of Salmonella epitope necessary for the production of the oral vaccine.
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KEYWORD
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Salmonella enterica serovar typhimurium, enteritidis, Invasion gene invH, invF and hilA cloning, Salmonella enterica serovar typhimurium, enteritidis
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